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UKAS Accredited Tests/Methods
Revision no: 4.0—Issue date 18 April 2008
Haematology | Immunoserology | Biochemistry | Endocrinology | Microbiology
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Sample Type
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Test
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Results Generated
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Equipment/Method
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Haematology:
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Whole blood
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Full blood count (including differential white blood cell count and
blood film assessment)
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Red cells
Haemoglobin
Hct
MCV
MCH
MCHC
RDW
White cells
Neutrophils
Lymphocytes
Monocytes
Eosinophils
Basophils
Platelets
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WET-HAE-SOP-91 (ACTS) and WET-HAE-SOP-92 (open tube) flow
cytometry (colorimetric haemoglobin) using Advia 120
WET-HAE-SOP-396 haemoglobin where necessary by colorimetric
analysis using Hemocue Spectrophotometer
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Morphological
assessment |
WET-HAE-SOP-399 and WET-HAE-SOP-401 manual film
assessment/differential using light microscope
WET-HAE-SOP-395 film stain using Hema Tek slide stainer
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Buffy coat
Preparation |
WET-HAE-SOP- 402 Buffy coat preparation where necessary, using
Hawksley microhaematocrit centrifuge and slide stainer
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Canine/Feline blood typing
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Canine/Feline blood type
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WET-HAE-SOP-405 (canine)
WET-HAE-SOP-406 (feline) using Dia Med Gel ID cards
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Canine/Feline Coombs test
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Canine Coombs test
Feline Coombs test
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WET-HAE-SOP-411 using VMRD canine Coombs kit
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Haematocrit
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Haematocrit (Hct)
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WET-HAE-SOP-394 by centrifugation�using Hawksley
microhaematocrit centrifuge
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Reticulocytes
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Reticulocytes
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*WET-HAE-SOP-371 by Supravital staining using the Advia 120/2120
WET-HAE-SOP-398 by Supravital staining and microscopy
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Whole blood and serum
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Cold agglutinins
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Cold auto-agglutinins
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WET-HAE-SOP-408 by haemagglutination and microscopy
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Cross-matching
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Compatibility result
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WET-HAE-SOP-407 by haemagglutination and microscopy
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Serum
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Antinuclear antibodies
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Antinuclear antibodies
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WET-HAE-SOP-409 by indirect immunofluorescence
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Equine PMSG
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PMSG 45–100 days
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WET-HAE-SOP-421 using DRG PMSG ELISA EIS
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Canine pregnancy test
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Canine pregnancy test
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WET-HAE-SOP-420 using Synbiotics
Reprocheck Relaxin ELISA
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Plasma (citrated)
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Fibrinogen
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Fibrinogen
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**WET-HAE-SOP-415 and WET-HAE-SOP-2210 by Clauss method using Helena AC4 automated coagulometer and Amelung KC10 coagulometer
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Prothrombin time
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Prothrombin time
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**WET-HAE-SOP-2210 and WET-HAE-SOP-413 by thromboplastin coagulation using Helena AC4 automated coagulometer and Amelung KC10 Coagulometer
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Partial thromboplastin time
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Partial thromboplastin time
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**WET-HAE-SOP-2210 and WET-HAE-SOP-414 by thrombosil coagulation using APTT-Si reagent on the Helena AC4 automated coagulometer and Amelung KC10 coagulometer
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Plasma (EDTA)
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Plasma viscosity
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Plasma viscosity
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WET-HAE-SOP-427 using Viscoliter
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*Advia 120 used for reticulocytes except where indicated as manual (via microscopy) on report
**Performed on Helena AC4 automated coagulometer unless otherwise identified on the report |
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Sample Type
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Test
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Results Generated
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Equipment/Method
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Immunoserology
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| Serum |
Heartworm, Lyme and Ehrlichia by SNAP® 3Dx®
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Heartworm antigen
Ehrlichia canis antibodies
Canine Lyme antibody
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WET-HAE-SOP-423 by IDEXX SNAP® 3Dx®
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FIV antibody detection
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FIV antibody test
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WET-HAE-SOP-418 by IDEXX ELISA
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FIV antibody detection
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FIV Western blot
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WET-HAE-SOP-419 by Western blot using IDEXX FIV kit
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FeLV antigen detection
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Feline leukaemia virus
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WET-HAE-SOP-417 using IDEXX FeLV ELISA
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Canine Pancreatic Lipase
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Canine Pancreatic Lipase Test
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WET-HAE-SOP-1928 using IDEXX CPLi kit
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Canine Anti-Thyroglobulin Antibodies
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TGAA Test
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WET-HAE-SOP-2139 by ELISA
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Canine Pro BNP
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Canine Pro BNP Test
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WET-HAE-SOP-2008 by ELISA
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Sample Type
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Test
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Results Generated
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Equipment/Method
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Biochemistry:
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Olympus 2700 and 640
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Serum/Plasma
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Albumin
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Albumin
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Photometric, bromocresol green WET-BIO-SOP-509
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Alkaline phosphatase
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Alkaline phosphatase 37C
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Photometric, IFCC EC 3.1.3.1
WET-BIO-SOP-510
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Alanine aminotransferase
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ALT (SGPT) 37C
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Photometric, IFCC without P5P
EC 2.6.1.2.
WET-BIO-SOP-512
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Aspartate aminotransferase
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AST(SGOT) 37C
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Photometric IFCC without
P5P EC 2.6.1.1
WET-BIO-SOP-513
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Amylase
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Amylase
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Photometric IFCC (EPS) EC 3.2.1.1
WET BIO-SOP-511
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B-hydroxy-butyrate
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BHB
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Photometric, kinetic:
WET-BIO-SOP-581
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Calcium
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Calcium
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Photometric, Arsenazo III
WET-BIO-SOP-517
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Cholesterol
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Cholesterol
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Photometric, CHOD-PAP
WET-BIO-SOP-518
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CK (CPK)
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CK (CPK) 37C
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Photometric, IFCC EC 2.7.3.2
WET-BIO-SOP-520
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Copper
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Copper
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Photometric
WET-BIO-SOP-1234
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Creatinine
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Creatinine
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Photometric, Jaffe kinetic
WET-BIO-SOP-525
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Total bilirubin
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Total bilirubin
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Photometric, modified O'Leary
WET-BIO-SOP-503
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Fructosamine
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Fructosamine
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Photometric, NBT reduction
WET-BIO-SOP-579
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Gamma GT
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Gamma GT
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Photometric, IFCC EC 2.3.2.2
WET-BIO-SOP-583
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Glucose
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Glucose
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Photometric HK G6P-DH
WET-BIO-SOP-528
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Glutamate dehydrogenase (GLDH)
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GLDH
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DGCK EC 1.4.1.3
WET-BIO-SOP-583
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Inorganic phosphorus
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Inorganic phosphorus
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Photometric, molybdate, UV
WET-BIO-SOP-529
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Lactate Dehydrogenase (LDH)
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LDH 37C
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Photometric, SCE EC 1.1.1.27
WET-BIO-SOP-534
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Lipase
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Lipase
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Photometric, enzymatic, colorimetric
WET-BIO-SOP-535
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Magnesium
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Magnesium
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Photometric, xylidyl blue
WET-BIO-SOP-536
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Total protein
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Total protein
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Photometric, blue violet
WET-BIO-SOP-539
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Sodium, potassium and chloride
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Sodium, potassium and chloride
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Indirect ISE
WET-BIO-SOP-496
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Triglycerides
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Triglycerides
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Photometric
WET-BIO-SOP-537
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Uric acid
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Uric acid
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Photometric, Uricase PAP
WET-BIO-SOP-541
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Urea
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Urea
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Photometric, Urease- GLDH
WET-BIO-SOP-540
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Bile acids
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Bile acids
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Photometric, L3K enzymatic recycling assay
WET-BIO-SOP-580
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Iron
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Iron
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Photometric, TPTZ
WET-BIO-SOP-530
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Urine
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Sodium, potassium and chloride
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Sodium, potassium and chloride
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Indirect ISE
WET-BIO-SOP-496
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Calcium
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Calcium
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Photometric, Arsenazo III
WET-BIO-SOP-517
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Creatinine
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Creatinine
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Photometric, Jaffe kinetic
WET-BIO-SOP-525
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Glucose
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Glucose
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Photometric HK G6P-DH
WET-BIO-SOP-528
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Inorganic phosphate
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Inorganic phosphate
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Photometric, molybdate, UV
WET-BIO-SOP-529
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Uric acid
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Uric acid
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Photometric, Uricase PAP
WET-BIO-SOP-541
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Urea
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Urea
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Photometric, Urease-GLDH
WET-BIO-SOP-540
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Urine/CSF
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Total Protein
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Total Protein
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Photometric, pyrogallol red/molybdate
WET-BIO-SOP-1700
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Helena SPIFE 3000 Analyser
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Serum
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Serum protein electrophoresis
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SPE
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WET-BIO-SOP-596 by agarose gel electrophoresis
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LDH isoenzymes
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LDH isoenzymes
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WET-BIO-SOP-597 by agarose gel electrophoresis
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Sample Type
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Test
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Results Generated
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Equipment/Method
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Endocrinology:
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Immulite 2000
Chemiluminescent competitive immunoassay
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Serum/Plasma
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Cobalamin
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Cobalamin
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WET-BIO-SOP-499
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Cortisol
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Cortisol
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WET-BIO-SOP-498
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Folate
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Serum folate
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WET-BIO-SOP-500
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Phenobarbitone
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Phenobarbitone
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WET-BIO-SOP-502
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Thyroxine
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Thyroxine
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WET-BIO-SOP-497
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Progesterone
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Progesterone
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Chemiluminescent immunometric assay WET-BIO-SOP-501
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Immulite 1
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Canine serum TSH
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Canine serum TSH
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Chemiluminescent immunometric assay
WET-BIO-SOP-443
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Canine Thyroxine
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Thyroxine
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Chemiluminescent competitive assay
WET-BIO-SOP-449
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Digoxin
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Digoxin
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Chemiluminescent competitive assay
WET-BIO-SOP-448
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Sample Type
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Test
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Results Generated
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Equipment/Method
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Microbiology:
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Animal swabs, tissues and biological fluids, as specified
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Swabs from eyes, nasal and throat, ears, skin, abscess/cyst, anal gland, interdigital cyst, pre-breeding, genitals
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Culture, isolation & antibiotic sensitivity of significant isolates
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Micro-organisms of veterinary clinical significance
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WET-MIC-SOP-664, 665, 666, 667, 668, 669, 670, 671 & 672 as appropriate to the sample type
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Equine Genital Tract Samples
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Screening for Taylorella equigenitalis, Klebsiellapneumoniae & pseudomonas aeroginosa
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Presence of Taylorella equigenitalis, Klebsiella pneumoniae and Pseudomonas aeruginosa
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WET-MIC-SOP-674 as screening for CEMO under the Code of Practice of the Horse Race Betting Levy Board
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Blood cultures
Fluids/Aspirates
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Culture, isolation & antibiotic sensitivity of significant isolates in blood cultures, fluids/ aspirates
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Micro-organisms of veterinary clinical significance
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WET-MIC-SOP-675
WET-MIC-SOP- 676 As appropriate for the sample type
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Aerobic and Microaerobic Organisms Isolated from Disease Situations
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Bacterial antibiotic sensitivity
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Susceptibility profile
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WET-MIC-SOP-717 using VITEK
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Skin, hair and skin samples
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Demonstrate presence of micro-organisms of veterinary clinical significance including bacteria, fungi, yeasts & ectoparasites
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WET-MIC-SOP-665
WET-MIC-SOP-673 As appropriate to the sample type
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Unspecified
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Zn staining
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Acid Fast Bacilli (AFB)
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WET-MIC-SOP-709 by Ziehl-Neelson staining
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Tissue Smears
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MZN staining
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Presence of Chlamydia psittaci (presumptive)
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WET-MIC-SOP-710 by modified Ziehl-Neelson staining
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Urine
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Urine examination
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Urine chemistry, white blood cells, red blood cells, crystals, casts & epithelial cells
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WET-MIC-SOP-692 by microscopy & dipsticks
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Urine examination
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Micro-organisms of veterinary clinical significance
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WET-MIC-SOP- 692
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Urine examination
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Specific Gravity
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WET-MIC-SOP-692 by refractometer
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Faeces
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Faecal examination, culture & identification
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Salmonella spp
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WET-MIC-SOP-650 by selective enrichment (Rappaports V broth) and selective plating (Brilliant Green Agar)
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Faecal examination, culture & identification
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Campylobacter spp
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WET-MIC-SOP-651 by direct plating
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Faecal examination
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Demonstration of parasites of veterinary clinical significance
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WET-MIC-SOP-653 using direct microscopy and NaCl flotation and WET-MIC-SOP-751 using ZnSO4 flotation
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Faecal examination
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Lungworm
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WET-MIC-SOP-654 by the Baermann test
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Faecal examination
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Cryptosporidium
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WET-MIC-SOP-655 by modified Ziehl-Neelson staining
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Faecal examination
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Worm egg count
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WET-MIC-SOP-656 using direct microscopy
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