Colilert®-18

Coliform/E. coli Results in 18 Hours

Colilert-18 is a test used for the detection of coliforms and E.coli in drinking water that gives you results in just 18 hours.

 

With Colilert-18, you can:

  • Read afternoon samples the next morning—before the next day's samples arrive
  • Lift boil water alerts in 18 hours
  • Provide results in record time for real estate, new well, and new construction samples
  • Read Friday samples early Saturday, leaving the rest of the weekend free

 

 

 

Colilert-18 Product Specifications
(sample size, shelf life, uses, and packaging)

Comparison of IDEXX Coliform and E. coli tests

Patent Information

Colilert-18

IDEXX products are accepted and approved in over 40 countries.

Colilert®-18 is easy to use, rapid and accurate

 

Easy
  • Ease of use simplifies training.
  • Unit-dosed packaging eliminates media preparation.
  • No repeat testing due to clogged filters or heterotrophic interference.
  • Quality Control (QC) procedure can be done in 15 minutes.

Rapid
  • Under one minute hands-on time.
  • Detects coliforms and E. coli simultaneously in 18 hours or less.
  • No confirmations needed.
  • No glassware cleaning or colony counting.

 

Accurate
  • Identifies E. coli specifically, which eliminates unneeded public notification due to Klebsiella pneumoniae.
  • Suppresses up to 2 million heterotrophs per 100 mL.
  • Eliminates the subjective interpretation found in traditional methods.
  • Detects a single viable bacterium per sample.

 

Economical
  • 95% less equipment costs than membrane filtration (MF).
  • Minimizes evening and weekend work.
  • Up to 15-month shelf life at room temperature.

 

Flexible
  • A Colilert®-18 Snap Pack can be used for presence/absence (P/A) or quantification testing.
  • Quanti-Tray® provides counts to 200/100 mL without dilutions.
  • Quanti-Tray®/2000 provides counts to 2,419/100 mL without dilutions.

Scientific Basis: How Colilert®-18 Works

 

Colilert-18 uses Defined Substrate Technology® (DST®) nutrient indicators ONPG and MUG to detect coliforms and E. coli. Coliforms use their β-galactosidase enzyme to metabolize ONPG and change it from colorless to yellow.

 

 

E.coli use β-glucuronidase to metabolize MUG and create fluorescence.

 

 

All IDEXX DST reagents can be used in a presence/absence (P/A) vessel, Quanti-Tray® or Quanti-Tray®/2000.

Colilert-18 Accessories

How to Use Colilert®-18

 
Presence / Absence (P/A)
Step 1.
Add reagent to sample and incubate 18 hours.
Step 2.
Read results:
  • Colourless = negative
  • Yellow = total coliforms
  • Yellow/fluorescent = E. coli
Quantification
Step 1.
Add reagent to sample.
Step 2. 
Pour into Quanti-Tray® (counts from 1–200) or Quanti-Tray®/2000 (counts from 1–2,419).
Step 3.
Seal in Quanti-Tray® Sealer and place in 35±0.5°C incubator for 18 hours (in other countries, the temperature requirement may be different per regulatory requirements).
View the fecal coliform procedure.
Step 4.
Quanti-Tray
—Read results:
  • Yellow wells = total coliforms
  • Yellow/fluorescent wells = E. coli
Quanti-Tray/2000—Read results:
  • Yellow wells = total coliforms
  • Yellow/fluorescent wells = E. coli

Colilert-18 Questions and Answers

 

Why is the Colilert-18 product irradiated?

We offer low-level gamma-irradiated Colilert-18 product to improve quality and eliminate possible contamination from coliform bacteria. View FAQs on irradiation.

 

What is the detection limit of Colilert-18?

Colilert-18 detects coliforms and E. coli at 1 organism/100 mL.

 

What is the shelf life of Colilert-18?

Colilert-18 shelf life is up to 15 months from date of manufacture.

 

What color and consistency should the Colilert-18 powder be?

The powder should be dry and free flowing. A slight tinge of color may be observed when Colilert-18 is added to the water sample, but this in no way interferes with the "readability" of the test. Any concerns about the color or integrity of the powder should be directed to IDEXX Technical Service.

 

Do Colilert-18 samples need to be prewarmed?

Yes, presence/absence (P/A) samples need to be prewarmed; however, Quanti-Tray® or Quanti-Tray®/2000 samples do not. For P/A, if the sample is not already at 33–38°C, place the sample in a 35°C waterbath for 20 minutes or a 44.5°C waterbath for 7–10 minutes. This prewarming time is part of (not in addition to) the 18-hour incubation period.

 

Can the Colilert-18 test be read beyond 18 hours?

Yes, if an inoculated Colilert-18 sample is inadvertently incubated beyond 18 hours, the result is stable for 4 additional hours (22 hours maximum incubation). Heterotrophic organisms are suppressed during the incubation. After 22 hours, a negative test is valid, but any yellow color greater than or equal to the comparator is not valid and should be repeated or verified.

 

Can I quantify my Colilert-18 results?

Yes, Colilert-18 can be run with Quanti-Tray®, Quanti-Tray/2000® or any multiple tube format.

 

What are the disposal requirements for a Colilert-18 sample?

Check with your local, state and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.

 
Resource List

How to Use Colilert-18 Video
How to Use Quanti-Tray Video
How to Use Quanti-Tray/2000 Video


 

Resource Library for Colilert®-18

 

 

General  •  Procedure  •  Technical  •  Approvals  •  U.S. EPA Approvals  •  MSDS
International Documents:  Dansk •  Deutsch •  Español •  Français •  Italiano •  Nederlands •  Português


Document TitleDocument TopicPDF Size

General
 
Water Products CatalogProduct Information 2.2 MB
Colilert-18 BrochureProduct Information 148 KB
IDEXX Vessel BrochureProduct Information 131 KB
IDEXX Sample Collection Vessel (PET) Information SheetProduct Information131 KB
Quanti-Tray BrochureProduct Information 346 KB
Quanti-Tray vs. Membrane FiltrationProduct Information 602 KB
IDEXX vs. Membrane Filtration Comparison GuideProduct Information 307 KB
Quanti-Tray/2000 vs. Multiple Tube FermentationProduct Information 225 KB
Quantification Method for Colilert, Colilert-18, Colisure and EnterolertProduct Information 132 KB
Quanti-Cult Information SheetProduct Information208 KB
Comparison of IDEXX Water Tests vs. CompetitorsProduct Information446 KB
 
 
Procedure
 
Colilert-18 ProcedureProcedure 443 KB
Quanti-Tray ProcedureProcedure 612 KB
Quanti-Tray/2000 ProcedureProcedure 1.35 MB
Quanti-Tray Sealer ProcedureProcedure 1.01 MB
Quanti-Cult Quality Control ProcedureProcedure 911 KB
Quanti-Tray Sealer Preventive Maintenance InstructionsProcedure 609 KB
15-Tube Most Probable Number Dilution ProcedureProcedure 69 KB
 
 
Technical
 
ISO/TR 13843 Method Performance Validation for Colilert-18/Quanti-TrayDrinking Water Studies  444 KB
A Simpler Method for Measuring Fecal Coliforms in WastewaterNon-Drinking Water Studies  710 KB
Colilert, Colilert-18 and Enterolert vs. Swedish Methods for Drinking and Bathing Waters, AEM. August, 1998 (Eckner, K.F.)Drinking Water Studies  10 KB
DST AOAC Full Collaborative Study. January/February 1991 (Edberg, Allen & Smith)Drinking Water Studies  11 KB
Enterolert, Colilert and Colilert-18 vs. UK Methods. November, 1995 (Fricker et al.)Drinking Water Studies  10 KB
UK Colilert-18 Quanti-Tray Evaluation "SCA Blue Book". October, 1998 (Barrell et al.)Drinking Water Studies  10 KB
UK PHLS Membrane Filtration vs. Colilert-18/Quanti-Tray Study. April 1999 (Graham, C.)Drinking Water Studies  9 KB
Colilert-18 vs. mF and mFC in Source Water (Ostensvik, O.)Non-Drinking Water Studies  5.6 MB
Heterotroph Article. February, 1989 (Edberg & Smith)Accuracy  9 KB
Membrane Filtration Quality Problems. January, 1990 (Brenner & Rankin)Competitive Methods  9 KB
Colilert vs. UK Standard Methods. 1996 (Fricker et al.)Competitive Methods  12 KB
Membrane Filtration Difficulties. May, 1986 (Jacobs et al.)Competitive Methods  9 KB
Water-borne Klebsiella and Human Disease. (Duncan, I.B.R.)Fecal Coliforms Vs. E. coli  10 KB
The Fecal Coliform Test vs. E. coli, IDEXX White Paper (Cummings, D.)Fecal Coliforms Vs. E. coli  11 KB
A Comparison of the International Standards Organisation Reference Method for the Detection of Coliforms and Escherichia coli in Water with a Defined Substrate ProcedureE. coli in Water 96 KB
 
 
U.S. EPA Approvals
 
U.S. EPA Approval Letter, Quanti-Tray. September, 1995.Approval 837 KB
U.S. EPA Approval of Colilert, Colilert-18, Colisure and Enterolert for Groundwater. August, 2006Approval 26 KB
U.S. EPA Approval of Colilert, Colilert-18, Enterolert, Quanti-Tray and Quanti-Tray/2000 for Ambient Water Testing. July, 2003Approval 184 KB
U.S. EPA Approval, Colilert-18 and Quanti-Tray/2000. September, 1996Approval 91 KB
U.S. EPA final approval of Colilert, Colilert-18, and Enterolert for Wastewater. March 2007Approval 337 KB
U.S. EPA LT2 Approval of Colilert, Colilert-18, Filta-Max, and Filta-Max xpress. January, 2006Approval 26 KB
U.S. EPA to Recommend Colilert-18 Inclusion in 40 CFR 136.3 for the Measurement of Fecal Coliforms in WastewaterApproval 568 KB
 
 
Approvals
 
Association Française de Normalisation (AFNOR) Validation Mark Granted for Colilert-18 and Quanti-Tray for Testing Drinking Water SamplesApproval 22 KB
Brazil Approval, Colilert, Colilert-18, Colisure, Quanti-Tray. January 19, 1990Approval 511 KB
Colilert, Colilert-18, Colisure Approval for Pharmaceutical Purified Water. January, 1985Approval 2.1 MB
Colilert-18 and Quanti-Tray Introduced as Reference Methods for Water Testing in Italy. November, 2004Approval 101 KB
Dairy Waters (Source Waters): FDA Approval as Both a Presence/Absence and Multiple-Tube Presence/Absence MethodApproval 225 KB
Denmark Approval: Colilert-18 and Quanti-Tray. February, 2005Approval 147 KB
FDA Approval for Colilert, Colilert-18 and Colisure and Quanti-Tray/Quanti-Tray/2000 for Bottled Water Source Water TestingApproval 190 KB
German Approval for Colilert-18/Quanti-Tray. December, 2002Approval 164 KB
IBWA Model Code Approval, Colilert, Colilert-18 and Colisure. 1999Approval 10 KB
Korean Approval for Colilert: Revisions to Official Drinking Water Quality Testing Methods. June, 2002Approval 252 KB
UK Approval of Colilert-18 and Quanti-Tray, Microbiology of Drinking Water 2002Approval 32 KB
Validation Study of the Colilert-18/Quanti-Tray Method for the Enumeration of Escherichia coli and Coliforms in Water for Human ConsumptionApproval 171 KB
WHO Recommendation of APHA Standard Methods, 1996Approval 706 KB
Standard Methods for the Examination of Water and Wastewater, 21st Edition, 2005, 9223 Enzyme Substrate Coliform TestApproval 21 KB
 
 
MSDS
 
Colilert-18 Material Safety Data SheetMaterial Safety Data Sheet 29 KB
Colilert Comparator Material Safety Data SheetMaterial Safety Data Sheet 41 KB
Quanti-Cult Material Safety Data SheetMaterial Safety Data Sheet 47 KB
Quanti-Tray Material Safety Data SheetMaterial Safety Data Sheet 34 KB
Vessel Material Safety Data SheetMaterial Safety Data Sheet 23 KB
Sterile Water Material Safety Data SheetMaterial Safety Data Sheet 132 KB
EZ-DPD Data SheetMaterial Safety Data Sheet 66 KB
 
 
Dokumente Auf Deutsch
 
Colilert-18 BroschüreProduktinformation (Deutsch) 1.4 MB
Colilert-18 VerfahrenVerfahren (Deutsch) 443 KB
Verwendung von Colilert-18 mit Quanti-Tray und Quanti-Tray/2000Produktinformation (Deutsch) 220 KB
Quanti-Tray BroschüreProduktinformation (Deutsch) 247 KB
Quanti-Tray VerfahrenVerfahren (Deutsch) 612 KB
Quanti-Tray Sealer VerfahrenVerfahren (Deutsch) 1.01 MB
Quanti-Tray Sealer ServiceanleitungVerfahren (Deutsch) 609 KB
Standardarbeitsanweisung (IDEXX SOP)Technical (Deutsch) 95 KB
Umweltbundesamt nimmt Colilert-18 / Quanti-Tray in die Liste zugelassener alternativer Trinkwassermethoden aufTechnical (Deutsch) 81 KB
Umweltbundesamt ruft zur Einreichung alternativer mikrobiologischer Untersuchungsverfahren auf (IDEXX submits Colilert-18/Quanti-Tray to German government)Technical (Deutsch) 13 KB
Italienisches Gesundheitsministerium nimmt Colilert-18 Quanti-Tray in die Liste alternativer Verfahren zur Trinkwasseranalyse aufTechnical (Deutsch) 24 KB
Publikation im UK DWI Report: Zulassung der britischen Umweltbehörde (IDEXX test published in UK DWI report)Technical (Deutsch) 9 KB
Colilert-18 MSDSMaterial Safety Data Sheet (Deutsch) 27 KB
Comparator MSDSMaterial Safety Data Sheet (Deutsch) 25 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Deutsch) 25 KB
Quanti-Cult MSDSMaterial Safety Data Sheet (Deutsch) 46 KB
Sterile Water MSDSMaterial Safety Data Sheet (Deutsch) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Deutsch)69 KB
 
 
Documentos en Español
 
ISO/TR 13843 Validación del método Colilert-18/Quanti-TrayAqua potable 492 KB
Folleto para Colilert-18Product Information (Español) 316 KB
Colilert-18 ProcedimientoProcedimiento (Español) 443 KB
Folleto para Quanti-TrayProduct Information (Español) 386 KB
Quanti-Tray ProcedimientoProcedimiento (Español) 612 KB
Cómo utilizar Colilert-18 con Quanti-Tray y Quanti-Tray/2000Procedimiento (Español) 220 KB
Quanti-Tray Sealer ProcedimientoProcedimiento (Español) 1.01 MB
Quanti-Tray Sealer Preventivo InstruccionesProcedimiento (Español) 609 KB
Hoja de datos de seguridad para Colilert-18Material Safety Data Sheet (Español) 25 KB
Hoja de datos de seguridad para ComparatorMaterial Safety Data Sheet (Español) 25 KB
Hoja de datos de seguridad de materiales para Quanti-TrayMaterial Safety Data Sheet (Español) 25 KB
Hoja de datos de seguridad de materiales para Quanti-CultMaterial Safety Data Sheet (Español) 47 KB
Hoja de datos de seguridad de materiales para sterile waterMaterial Safety Data Sheet (Español) 39 KB
Hoja de datos de seguridad para recipientes de poliestirenoMaterial Safety Data Sheet (Español) 115 KB
Hoja de datos de seguridad de materiales para EZ-DPDMaterial Safety Data Sheet (Español) 69 KB
 
 
Documents en Français
 
ISO/TR 13843 Validation de Colilert-18/Quanti-TrayEau potable 463 KB
Brochure pour Colilert-18Information sur le produit (Français) 323 KB
Brochure pour Quanti-TrayProduct Information (Français) 383 KB
Colilert Présence/Absence ProcedureProcédure: (Français) 292 KB
Colilert-18 ProcédureProcédure (Français) 443 KB
Quanti-Tray ProcédureProcédure: (Français) 612 KB
Quanti-Tray Sealer ProcédureProcédure: (Français) 1.01 MB
Quanti-Tray Sealer Maintenance Préventive InstructionsProcédure: (Français) 609 KB
Fiche de sécurité pour Colilert-18Material Safety Data Sheet (Français) 27 KB
Fiche de sécurité pour ComparatorMaterial Safety Data Sheet (Français) 27 KB
Fiche de sécurité pour Quanti-TrayMaterial Safety Data Sheet (Français) 25 KB
Fiche de sécurité pour Quanti-CultMaterial Safety Data Sheet (Français) 47 KB
Fiche de sécurité pour sterile waterMaterial Safety Data Sheet (Français) 39 KB
Fiche signalétique pour récipients en polystyrèneMaterial Safety Data Sheet (Français) 114 KB
Colilert-18/Quanti-Tray accepté en AllemagneApprobation (Français) 74 KB
Les méthodes IDEXX sont de plus en plus acceptées en EuropeApprobation (Français) 87 KB
Fiche de sécurité pour EZ-DPDMaterial Safety Data Sheet (Français) 68 KB
 
 
Documenti in Italiano
 
ISO/TR 13843 Validazione di Colilert-18/Quanti-TrayAcqua potabile 449 KB
Opusculo per Colilert-18Informazioni di prodotto (Italiano) 323 KB
Colilert-18 ProceduraProcedura (Italiano) 443 KB
Opusculo per Quanti-TrayInformazioni di prodotto (Italiano) 389 KB
Quanti-Tray ProceduraProcedura (Italiano) 612 KB
Come utilizzare Colilert-18 con Quanti-Tray e Quanti-Tray/2000Procedura (Italiano) 196 KB
Quanti-Tray Sealer ProceduraProcedura (Italiano) 1.01 MB
Quanti-Tray Sealer Manutenzione Preventiva IstruzioniProcedura (Italiano) 609 KB
Il Ministero della Sanita'Italiano Inserisce Colilert-18 e Quanti-Tray Nell'elenco dei Metodi di Analisi Dell'acqua Potabile AlternativiApprovazione (Italiano) 25 KB
Colilert-18 MSDSMaterial Safety Data Sheet (Italiano) 25 KB
Comparator MSDSMaterial Safety Data Sheet (Italiano) 25 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Italiano) 25 KB
Quanti-Cult MSDSMaterial Safety Data Sheet (Italiano) 46 KB
Sterile Water MSDSMaterial Safety Data Sheet (Italiano) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Italiano) 67 KB
 
 
Documentos em Português
 
ISO/TR 13843 Validação do method Colilert-18/Quanti-TrayAcqua potabile 454 KB
Folheto para Colilert-18Product Information (Português) 318 KB
Colilert-18 MSDSMaterial Safety Data Sheet (Português) 56 KB
Colilert Comparator MSDSMaterial Safety Data Sheet (Português)25 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Português) 38 KB
Quanti-Cult MSDSMaterial Safety Data Sheet (Português) 46 KB
Sterile Water MSDSMaterial Safety Data Sheet (Português) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Português)72 KB
 
 
Documenten in het Nederlands
 
Colilert-18 MSDSMaterial Safety Data Sheet (Nederlands) 56 KB
Colilert Comparator MSDSMaterial Safety Data Sheet (Nederlands) 25 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Nederlands) 38 KB
Quanti-Cult MSDSMaterial Safety Data Sheet (Nederlands) 39 KB
Sterile Water MSDSMaterial Safety Data Sheet (Nederlands) 38 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Nederlands)68 KB
 
 
Dokumenter på dansk
 
Colilert-18 MSDSMaterial Safety Data Sheet (Danske) 61 KB
Colilert Komparator SikkerhedsdatabladMaterial Safety Data Sheet (Danske) 25 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Danske) 38 KB
Quanti-Cult MSDSMaterial Safety Data Sheet (Danske) 46 KB
Sterile Water MSDSMaterial Safety Data Sheet (Danske) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Danske)67 KB