Enterolert®

Enterococci Results in 24 Hours

 

Enterolert® detects and quantifies enterococci in just 24 hours.

Getting accurate results overnight lets you:

  • Discover public beach contamination in half the time of standard methods
  • Re-open beaches in just 24 hours
  • Eliminate tedious membrane filtration work

 

Enterolert is an official ASTM Method (#D6503-99).

  • Enterolert Product Specifications
    (sample size, shelf life, uses, and packaging)
  • Comparison of IDEXX Enterococci Tests
  • Patent Information
 

Enterolert® is easy to use, rapid and accurate

 

Easy
  • No media preparation
  • No autoclaving
  • No colony counting
  • No glassware cleaning

 

Rapid
  • Under one minute hands-on time
  • Results in 24 hours rather than 48 to 72 hours

 

Accurate
  • Sensitive to 1 enterococci/100 mL
  • Enumerates up to 2,419 enterococci per 100 mL without dilutions (with Quanti-Tray®/2000)
  • Less subjective interpretation
  • 50% fewer false positives and 95% fewer false negatives than the standard membrane filtration (MF) method1

 

Economical
  • 75% lower equipment cost than membrane filtration
  • Up to 12-month shelf life minimizes waste
  • 24-hour test saves incubator space

 

1
Budnick, GE, Howard, RT and Mayo, DR. Evaluation of Enterolert for Enumeration of Enterococci in Recreational Water. Applied and Environmental Microbiology. 1996;62:3881–3884.

Scientific Basis: How Enterolert® Works

 

Enterolert uses a Defined Substrate Technology® (DST®) nutrient-indicator to detect enterococci. This nutrient-indicator fluoresces when metabolised by enterococci. DST improves accuracy and avoids the need for hazardous sodium azide suppressants used in traditional media.

 

Enterolert Accessories

How to Use Enterolert®

 
Quantification

Step 1. 
Add reagent to sample.

Step 2.
Pour into Quanti-Tray® (counts from 1-200) or Quanti-Tray®/2000 (counts from 1–2,419).

Step 3.
Seal in Quanti-Tray® Sealer and incubate for 24 hours at 41°C.

Step 4.

Count fluorescent wells and refer to most probable number (MPN) table.

Quanti-Tray
(counts from 1 to 200)


Quanti-Tray/2000
(counts from 1 to 2,419)

 

Enterolert Questions and Answers

 

What species of enterococci can Enterolert detect?

Some species of enterococci that Enterolert detects are: faecalis, faecium, avium, gallinarum, casseliflavis, and durans.

 

What is the detection limit of Enterolert?

Enterolert detects enterococci at 1 organism/100 mL.

 

What is the shelf life of Enterolert?

Enterolert shelf life is up to 12 months from the date of manufacture.

 

What color and consistency should the Enterolert powder be?

The Enterolert reagent powder should be tan in color and free-flowing. Any concerns about the color or integrity of the powder should be directed to IDEXX Technical Service.

 

Can I quantify my Enterolert results?

Yes, Enterolert can be run with Quanti-Tray®, Quanti-Tray®/2000 or any multiple tube format.

 

What are the disposal requirements for an Enterolert sample?

Check with your local, state and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.

 
Resource List

How to Use Enterolert Video
How to Use Quanti-Tray Video
How to Use Quanti-Tray/2000 Video

 

Resource Library for Enterolert®

 

General •  Procedure •  Technical •  U.S. EPA Approvals •  Approvals •  MSDS
International Documents:  Dansk •  Deutsch •  Español •  Français •  Italiano •  Nederlands •  Português


Document TitleDocument TopicPDF Size
General
 
Water Products CatalogProduct Information 2.2 MB
IDEXX Bottled Water BrochureProduct Information 620 KB
Enterolert BrochureProduct Information 266 KB
IDEXX Vessel BrochureProduct Information 131 KB
IDEXX Sample Collection Vessel (PET) Information SheetProduct Information 131 KB
Quanti-Tray BrochureProduct Information 346 KB
Quanti-Tray vs. Membrane FiltrationProduct Information 602 KB
IDEXX vs. Membrane Filtration Comparison GuideProduct Information 307 KB
Quanti-Tray/2000 vs. Multiple Tube FermentationProduct Information 225 KB
Quantification Method for Colilert, Colilert-18, Colisure and EnterolertProduct Information 132 KB
Comparison of IDEXX Water Tests vs. CompetitorsProduct Information446 KB
back to the top
 
 
Procedure
 
Enterolert ProcedureProcedure 439 KB
Quanti-Tray ProcedureProcedure 612 KB
Quanti-Tray/2000 ProcedureProcedure 1.35 MB
Quanti-Tray Sealer ProcedureProcedure 1.01 MB
Quanti-Tray Sealer Preventive Maintenance InstructionsProcedure 609 KB
back to the top
 
 
Technical
 
Colilert, Colilert-18 and Enterolert vs. Swedish Methods for Drinking and Bathing Waters, AEM. August, 1998 (Eckner, K.F.)Drinking Water Studies  10 KB
Colilert and Enterolert Correlate Well with Membrane Filtration for Enumerating Fecal Coliforms in Wastewater (Yakub et al.)Non-Drinking Water Studies  574 KB
Enterolert, Colilert and Colilert-18 vs. UK Methods. November, 1995 (Fricker et al.)Drinking Water Studies  10 KB
AEM article 10/1996, Enterolert in Recreation Waters (Budnick et al.)Non-Drinking Water Studies  8 KB
ASM Poster 1996, Enterolert in Recreation Waters (Budnick et al.)Non-Drinking Water Studies  9 KB
Enterolert Initial Validation. November, 1995 (Chen et al.)Non-Drinking Water Studies  247 KB
MF's Low Accuracy For Enterococci. 1975 (Levin et al.)Non-Drinking Water Studies  8 KB
Membrane Filtration Quality Problems. January, 1990 (Brenner & Rankin)Competitive Methods  9 KB
Membrane Filtration Difficulties. May, 1986 (Jacobs et al.)Competitive Methods  9 KB
back to the top
 
 
U.S. EPA Approvals
 
U.S. EPA Wastewater Approval of Enterolert. November, 2007Approval 15 KB
U.S. EPA Approval Letter, Quanti-Tray. September, 1995.Approval 837 KB
U.S. EPA Approval of Colilert, Colilert-18, Colisure and Enterolert for Groundwater. November, 2006Approval 26 KB
U.S. EPA Approval of Colilert, Colilert-18, Enterolert, Quanti-Tray and Quanti-Tray/2000 for Ambient Water Testing. July, 2003Approval 184 KB
U.S. EPA final approval of Colilert, Colilert-18, and Enterolert for Wastewater. March, 2007Approval 337 KB
back to the top
 
 
Approvals
 
ASTM Approval D6503-99, Enterolert, December, 1999Approval 61 KB
Connecticut Enterolert Approval. July, 1996Approval 746 KB
Inclusion of Enterolert in Standard Methods for the Examination of Water and WastewaterApproval 84 KB
New Zealand Bathing Water Approval, Colilert and Enterolert. November, 1999Approval 72 KB
back to the top
 
 
MSDS
 
Enterolert Material Safety Data SheetMaterial Safety Data Sheet 41 KB
Quanti-Tray Material Safety Data SheetMaterial Safety Data Sheet 34 KB
Vessel Material Safety Data SheetMaterial Safety Data Sheet 23 KB
Sterile Water Material Safety Data SheetMaterial Safety Data Sheet 39 KB
EZ-DPD Data SheetMaterial Safety Data Sheet 66 KB
back to the top
 
 
Dokumente Auf Deutsch
 
Enterolert VerfahrenVerfahren (Deutsch) 439 KB
IDEXX Bottled Water BroschüreProduktinformation (Deutsch) 569 KB
Quanti-Tray BroschüreProduktinformation (Deutsch) 247 KB
Quanti-Tray VerfahrenVerfahren (Deutsch) 612 KB
Quanti-Tray Sealer VerfahrenVerfahren (Deutsch) 1.01 MB
Quanti-Tray Sealer ServiceanleitungVerfahren (Deutsch) 609 KB
Enterolert MSDSMaterial Safety Data Sheet (Deutsch) 25 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Deutsch) 25 KB
Sterile Water MSDSMaterial Safety Data Sheet (Deutsch) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Deutsch)69 KB
back to the top
 
 
Documentos en Español
 
Enterolert ProcedimientoProcedimiento (Español) 439 KB
IDEXX Folleto de agua embotelladaProcedimiento (Español) 593 KB
Folleto para Quanti-TrayInformación de Producto (Español) 386 KB
Quanti-Tray ProcedimientoProcedimiento (Español) 612 KB
Quanti-Tray Sealer ProcedimientoProcedimiento (Español) 1.01 MB
Quanti-Tray Sealer Mantenimiento Preventivo InstruccionesProcedimiento (Español) 609 KB
Hoja de datos de seguridad para EnterolertMaterial Safety Data Sheet (Español) 25 KB
Hoja de datos de seguridad de materiales para Quanti-TrayMaterial Safety Data Sheet (Español) 25 KB
Hoja de datos de seguridad de materiales para sterile waterMaterial Safety Data Sheet (Español) 39 KB
Hoja de datos de seguridad de materiales para EZ-DPDMaterial Safety Data Sheet (Español) 69 KB
back to the top
 
 
Documents en Français
 
Brochure IDEXX l'eau embouteilléeInformation sur le produit (Français) 600 KB
Enterolert ProcédureProcédure (Français) 439 KB
Brochure pour Quanti-TrayInformation sur le produit (Français) 383 KB
Quanti-Tray ProcédureProcédure (Français) 612 KB
Quanti-Tray Sealer ProcédureProcédure (Français) 1.01 MB
Quanti-Tray Sealer Maintenance Préventive InstructionsProcédure (Français) 609 KB
Fiche de sécurité pour EnterolertMaterial Safety Data Sheet (Français) 27 KB
Fiche de sécurité pour Quanti-TrayMaterial Safety Data Sheet (Français) 25 KB
Fiche de sécurité pour sterile waterMaterial Safety Data Sheet (Français) 39 KB
Fiche de sécurité pour EZ-DPDMaterial Safety Data Sheet (Français) 68 KB
back to the top
 
 
Documenti in Italiano
 
IDEXX Brochure Acqua in bottigliaInformazioni di prodotto (Italiano) 588 KB
Enterolert ProceduraProcedura (Italiano) 439 KB
Opusculo per Quanti-TrayInformazioni di prodotto (Italiano) 389 KB
Quanti-Tray ProceduraProcedura (Italiano) 612 KB
Quanti-Tray Sealer ProceduraProcedura (Italiano) 1.01 MB
Quanti-Tray Sealer Manutenzione Preventiva IstruzioniProcedura (Italiano) 609 KB
Enterolert MSDSMaterial Safety Data Sheet (Italiano) 25 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Italiano) 25 KB
Sterile Water MSDSMaterial Safety Data Sheet (Italiano) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Italiano) 67 KB
back to the top
 
 
Documentos em Português
 
IDEXX Brochura água engarrafadaInformações sobre o produto (Português) 624 KB
Quanti-Tray MSDSMaterial Safety Data Sheet (Português) 38 KB
Sterile Water MSDSMaterial Safety Data Sheet (Português) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Português)72 KB
back to the top
 
 
Documenten in het Nederlands
 
Quanti-Tray MSDSMaterial Safety Data Sheet (Nederlands) 38 KB
Sterile Water MSDSMaterial Safety Data Sheet (Nederlands) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Nederlands)68 KB
back to the top
 
 
Dokumenter på dansk
 
Quanti-Tray MSDSMaterial Safety Data Sheet (Danske) 38 KB
Sterile Water MSDSMaterial Safety Data Sheet (Danske) 39 KB
EZ-DPD MSDSMaterial Safety Data Sheet (Danske)67 KB
back to the top