The Colisure Test uses proprietary Defined Substrate Technology (DST) nutrient indicators CPRG and MUG to detect total coliforms and E. coli. Coliforms use the β-galactosidase enzyme to metabolise CPRG and change it from yellow to magenta. E. coli use β-glucuronidase to metabolise MUG and create fluorescence.
Add reagent to sample and incubate at 35 ± 0.5°C for 24 hours.
Add reagent to sample.
Seal in Quanti-Tray Sealer and place in incubator at 35 ± 0.5°C for 24 hours.
What is the detection limit of the Colisure Test ?
The Colisure Test detects coliforms and E. coli at 1 organism/100 mL.
What is the shelf life of the Colisure Test ?
The shelf life for the Colisure Test is up to 12 months from date of manufacture.
What are the recommended storage conditions for the Colisure Test ?
Store at 2–25°C and away from light.
Is there a comparator for the Colisure Test ?
There is no comparator for the Colisure Test. For comparison, use a negative control when interpreting results.
Why is the read window 24–48 hours?
The reagent system was designed to suppress non-coliform CPRG or MUG positive bacteria for at least 48 hours. If incubation extends past 48 hours, it is possible that such bacteria could overcome the reagent and react with CPRG or MUG. When incubation exceeds 48 hours, only negative results are valid. Positive results must be confirmed.
What are the disposal requirements for a Colisure Test sample?
Check with your local authority for proper disposal of bacteriological biohazard materials at your facility.
Can samples be read after 48 hours?
If an inoculated Colisure Test sample is inadvertently incubated over 48 hours, lack of red to magenta colour is a valid negative test; a red to magenta colour after 48 hours is not valid and should be repeated.
Can samples be read before 24 hours?
An innoculated Colisure Test sample that is positive for coliforms and E. coli before 24 hours is a valid positive test for both coliforms and E. coli. If the sample is positive before 24 hours for coliforms only, the sample is a valid positive test for total coliforms. However, the sample should be incubated for a longer period (at least 24 hours, but not greater than 48 hours total time) to determine whether E. coli are present.
Can I use the Colisure Test with Quanti-Tray and Quanti-Tray/2000?
Yes, when using the Colisure Test with Quanti-Tray, Quanti-Tray/2000 or any multiple-tube format, be sure to use IDEXX Antifoam Solution or a vessel with antifoam.
What should the colour and consistency of the Colisure reagent powder be?
The Colisure reagent powder should be tan in colour; it should be dry and free-flowing. Any concerns about the colour or integrity of the powder should be directed to IDEXX Technical Support.
Colisure Test (20-test pack)
Catalogue Number: WCLS20I
Colisure Test (100-test pack)
Catalogue Number: WCLS100I
Colisure Test (200-test pack)
Catalogue Number: WCLS200I
IDEXX Technologies Ltd Units 1B & 1C, Newmarket Business Park, Studlands Park Avenue, Newmarket, Suffolk, CB87ER
Tel: 01638 676800