Accurately detects enterococci in 24 hours
Detects enterococci in drinking water; 100 ml format. Results in 24 hours.
The Enterolert®-DW Test, coupled with the Quanti-Tray® system, provides quantitative confirmed results in 24 hours.
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Enterolert-DW: Be confident in your outcomes
How Enterolert®-DW works
Enterolert-DW uses a Defined Substrate Technology® (DST®) nutrient indicator to detect enterococci. When coupled with the IDEXX Quanti-Tray® System, Enterolert-DW provides quantitative confirmed results in 24 hours. Enterolert-DW utilises ortho-nitrophenyl-β-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background colour in its formulation. When the enzyme substrate is metabolised by enterococci, the sample turns from blue to green to indicate detection. Any change from the original colour to green is considered a positive result. No ultraviolet light source is required.
A step-by-step guide to using Enterolert-DW
Add reagent to sample.
Cap vessel and shake to mix.
Seal using Quanti-Tray® Sealer and incubate at 41 ±0.5°C for 24 hours.
Count positive wells and refer to most probable number (MPN) table.
Frequently asked questions and answers
Enterolert-DW is designed to detect Enterococcus species in drinking water samples. It is based on IDEXX’s patented Defined Substrate Technology® (DST®). Enterolert-DW utilizes ortho-nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation. When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive detection. Any change from the original color to green is considered a positive result. No ultraviolet light source is required.
Enterolert-DW is designed to detect enterococci in drinking water. As with any microbial method, some Enterococcus species are more easily detected than others. Some examples of Enterococcus species we have detected in trials and studies:
There is no comparator for Enterolert-DW. For comparison, use a negative control when interpreting results.
Enterolert-DW is incubated at 41°C ± 0.5°C for 24 hours and the results are valid up to 28 hours, unless your local regulations indicate differently.
Yes, if they are positive. An inoculated Enterolert-DW sample that is positive for enterococci before 24 hours is a confirmed positive test for enterococci.
Yes, if they are negative. If an inoculated Enterolert-DW sample is inadvertently incubated over 28 hours, lack of green color is a valid negative test; a green color after the 28 hours is not valid and should be repeated or verified.
Because Enterolert-DW is used with the Quanti-Tray system, any sterile vessel can be used.
Store Enterolert-DW at 2–25°C, away from direct light and humidity.
When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive result. Any change from the original color to green is considered a positive result.
Enterolert-DW is designed specifically for drinking water testing. Extensive studies were performed on enterococci stressed by chlorine, to mimic the impact of water treatment.
Enterolert-DW may be used for other water types. Before implementing testing, IDEXX recommends you use a typical laboratory validation protocol to assure that any other water types will work with this product before implementation.
The Enterolert-DW reagent powder should be white to off-white in colour with a slight greenish tint; it should be dry and free-flowing. Any concerns about the colour or integrity of the reagent powder should be directed to IDEXX Technical Support at +1 800 321 0207 or via email at firstname.lastname@example.org.