Enterolert®-DW

Accurately detects enterococci in 24 hours

 
Drinking Water Bottled Water
 

Detects enterococci in drinking water; 100 ml format. Results in 24 hours.

 

The Enterolert®-DW Test, coupled with the Quanti-Tray® system, provides quantitative confirmed results in 24 hours.

 

Which IDEXX enterococci test is right for you? View a comparison.

 

 

What's New

AFNOR validates Enterolert®-DW for detection of enterococci in drinking water.

Enterolert-DW
 

Questions?

Call 01638 676800 or email water@idexx.com.

Enterolert-DW: Be confident in your outcomes

 

 

Easy

  • Ready-to-use media.
  • Simple procedure.
  • Most Probable Number (MPN) enumeration.
  • Unit-dosed packaging eliminates media preparation.
  • Reagent contains no toxic compounds.
 

Rapid

  • Confirmed results in 24 hours.
  • Simplified quality control.
  • Less than 1 minute of hands-on time.
 

Accurate

  • More accurate when compared to ISO 7899-2 on all samples.
  • More accurate when compared to Microbiology of Drinking Water (MoDW) 2006, part 5, on chlorine-stressed samples.
  • Liquid enrichment ensures optimised recovery.
  • Eliminates subjective interpretation of results determined by traditional methods.
Resource List
 
 

How Enterolert®-DW works

 

Enterolert-DW uses a Defined Substrate Technology® (DST®) nutrient indicator to detect enterococci. When coupled with the IDEXX Quanti-Tray® System, Enterolert-DW provides quantitative confirmed results in 24 hours. Enterolert-DW utilises ortho-nitrophenyl-β-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background colour in its formulation. When the enzyme substrate is metabolised by enterococci, the sample turns from blue to green to indicate detection. Any change from the original colour to green is considered a positive result. No ultraviolet light source is required.

 

 

View/Print Enterolert-DW Procedure 

 
 
Learn how to use Enterolert/Enterolert-E

 

 

 

 
 
Learn how to use Quanti-Tray

 

 

 

 
 
Learn how to use Quanti-Tray/2000

 

 

 

 

 

A step-by-step guide to using Enterolert-DW

 
Quantification
 
 

Step 1

Add reagent to sample.

 
   
 

Step 2

Cap vessel and shake to mix.

   
 

Step 3

Add sample reagent to Quanti-Tray® (counts from 1–200) or Quanti-Tray®/2000 (counts from 1–2,419).

   
 

Step 4

Seal using Quanti-Tray® Sealer and incubate at 41 ±0.5°C for 24 hours.

   
 

Step 5

Count positive wells and refer to most probable number (MPN) table.

 

Frequently asked questions and answers

 

How does the product work?

Enterolert-DW is designed to detect Enterococcus species in drinking water samples. It is based on IDEXX’s patented Defined Substrate Technology® (DST®). Enterolert-DW utilizes ortho-nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation. When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive detection. Any change from the original color to green is considered a positive result. No ultraviolet light source is required.

 

What species of enterococci can Enterolert-DW detect?

Enterolert-DW is designed to detect enterococci in drinking water. As with any microbial method, some Enterococcus species are more easily detected than others. Some examples of Enterococcus species we have detected in trials and studies:

Enterococcus faecalis
Enterococcus faecium
Enterococcus hirae
Enterococcus gallinarum
Enterococcus casseliflavus
Enterococcus durans
Enterococcus avium

 

Is there a comparator for Enterolert-DW?

There is no comparator for Enterolert-DW. For comparison, use a negative control when interpreting results.

 

What is the incubation time and temperature of Enterolert-DW?

Enterolert-DW is incubated at 41°C ± 0.5°C for 24 hours and the results are valid up to 28 hours, unless your local regulations indicate differently.

 

Are results valid prior to 24 hours?

Yes, if they are positive. An inoculated Enterolert-DW sample that is positive for enterococci before 24 hours is a confirmed positive test for enterococci.

 

Are results valid after 28 hours?

Yes, if they are negative. If an inoculated Enterolert-DW sample is inadvertently incubated over 28 hours, lack of green color is a valid negative test; a green color after the 28 hours is not valid and should be repeated or verified.

 

Which vessels should I use with Enterolert-DW?

Because Enterolert-DW is used with the Quanti-Tray system, any sterile vessel can be used.

 

What are the recommended storage conditions for Enterolert-DW?

Store Enterolert-DW at 2–25°C, away from direct light and humidity.

 

What normal color change should take place with Enterolert-DW?

When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive result. Any change from the original color to green is considered a positive result.

 

What water types can the product be used on/what water type is the product designed to be used for?

Enterolert-DW is designed specifically for drinking water testing. Extensive studies were performed on enterococci stressed by chlorine, to mimic the impact of water treatment.

 

Can Enterolert-DW be used on water types other than drinking water?

Enterolert-DW may be used for other water types. Before implementing testing, IDEXX recommends you use a typical laboratory validation protocol to assure that any other water types will work with this product before implementation.

 

What color and consistency should the Enterolert-DW reagent powder be?

The Enterolert-DW reagent powder should be white to off-white in colour with a slight greenish tint; it should be dry and free-flowing. Any concerns about the colour or integrity of the reagent powder should be directed to IDEXX Technical Support at +1 800 321 0207 or via email at water@idexx.com.

 

Enterolert-DW: Everything you need for confident outcomes

 
 

Resource Library for Enterolert-DW

 

View Resource Library for all products.