Multiple Enzyme Technology Medium—the SimPlate for HPC medium uses IDEXX Multiple Enzyme Technology (MET) to detect heterotrophic plate count (HPC) bacteria in water. The reagent contains multiple unique enzyme substrates, each targeting a different bacterial enzyme. The most common enzymes of waterborne bacteria are all targeted.
All of the enzyme substrates produce the same signal when metabolised. Therefore, a given waterborne bacteria will be able to metabolise one or more enzyme substrates and produce a signal. In the case of SimPlate for HPC, the signal is fluorescence under 365 nm UV light.
After the sample and medium are added to the plate, the wells fill automatically as the user swirls the plate. No pipetting is needed.
Once the wells are filled, the plate is angled and excess sample/medium mixture is poured into the absorbent pad. The SimPlate takes advantage of surface tension to hold the proper amount of sample/medium mixture in each of its 84 wells as excess is poured off.
SimPlates are incubated upside down to prevent condensation from collecting on the lid. Since each well is isolated from the others, bacteria from one well will cause only that one well to fluoresce.
Add 100 mL sterile diluent to media vessel.
Add 1 mL sample to SimPlate.
Add 9 mL media to SimPlate.
Swirl SimPlate and pour off excess.
Invert and incubate for 48 hours.
Read results under UV light.
The shelf life is up to 15 months from date of manufacture.
What are the recommended storage conditions for SimPlate for HPC?
Store at 2°C–30°C and away from light.
Are SimPlates sterile?
Yes, the base and lid are gamma irradiated. There are 25 plates per plastic sleeve. Any unused plates should remain in the bag and the bag should be closed. Do not leave any unused plates exposed to the environment.
Are SimPlates made of recyclable plastic?
The bottom portion of the plate is made of polystyrene #6 and is recyclable. The cover/lid is made of polyvinyl chloride and is not recyclable.
IDEXX Water has reference materials and approval documents to support the many products in our water portfolio.
SimPlate for HPC, unit-dose kit (25 tests)
Catalogue number: WHPC-25
SimPlate for HPC, multi-dose kit (100 tests, 10 media vessels and SimPlates)
Catalogue number: WHPC-100
SimPlate for HPC, media-only kit (40 tests)
Catalogue number: WHPC-40M
IDEXX Technologies Ltd Units 1B & 1C, Newmarket Business Park, Studlands Park Avenue, Newmarket, Suffolk, CB87ER
Tel: 01638 676800
1. Eaton AD, Clesceri LS, Greenberg AE, eds. Standard Methods for Examination of Water and Wastewater. 22nd ed. Washington, DC: American Public Health Association: 2012.
2. Data on file at IDEXX Laboratories, Inc.