SimPlate for HPC Test

Heterotrophic plate counts, simplified

Simplate for HPC main image

   
Easy-to-count wells on a round plate that correlates to plate count agar. Positives fluoresce under UV light.

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Overview

Easy
  • Easier to read and use than the pour-plate method.
  • No agar preparation.
  • Most Probable Number (MPN) counting range minimises need for dilutions.
Rapid
  • Instant media preparation.
  • Less than 2 minutes of hands-on time.
  • Reduced read time.
Accurate
  • Simplified procedure minimises chance of contamination.
  • Less subjective interpretation than pour plate.
Economical
  • Less equipment to purchase and maintain.
  • Increased productivity saves labour costs.
  • Minimal Quality Control (QC).

Science

How SimPlate for HPC works

Multiple Enzyme Technology Medium—the SimPlate for HPC medium uses IDEXX Multiple Enzyme Technology (MET) to detect heterotrophic plate count (HPC) bacteria in water. The reagent contains multiple unique enzyme substrates, each targeting a different bacterial enzyme. The most common enzymes of waterborne bacteria are all targeted.

All of the enzyme substrates produce the same signal when metabolised. Therefore, a given waterborne bacteria will be able to metabolise one or more enzyme substrates and produce a signal. In the case of SimPlate for HPC, the signal is fluorescence under 365 nm UV light.

After the sample and medium are added to the plate, the wells fill automatically as the user swirls the plate. No pipetting is needed.

Once the wells are filled, the plate is angled and excess sample/medium mixture is poured into the absorbent pad. The SimPlate takes advantage of surface tension to hold the proper amount of sample/medium mixture in each of its 84 wells as excess is poured off.

SimPlates are incubated upside down to prevent condensation from collecting on the lid. Since each well is isolated from the others, bacteria from one well will cause only that one well to fluoresce.

The number of wells that fluoresce is converted to a Most Probable Number (MPN) using the table provided with the product. The high number of wells (84) results in a very high counting range and precise 95% confidence limits. The counting range is from <1 to 738 per plate (more than double a standard pour plate) with the multi-dose SimPlate for HPC.



How to Use

How to use SimPlate for HPC

Step-by-step guide to using SimPlate for HPC

Instructions

Step 1

Add 100 mL sterile diluent to media vessel.

Step 2

Add 1 mL sample to SimPlate.

Step 3

Add 9 mL media to SimPlate.

Step 4

Swirl SimPlate and pour off excess.

Step 5

Invert and incubate for 48 hours.

Step 6

Read results under UV light.

 

Q & A

Frequently asked questions and answers
What is the shelf life of SimPlate for HPC?

The shelf life is up to 15 months from date of manufacture.

What are the recommended storage conditions for SimPlate for HPC?
Store at 2°C–30°C and away from light.

Are SimPlates sterile?
Yes, the base and lid are gamma irradiated. There are 25 plates per plastic sleeve. Any unused plates should remain in the bag and the bag should be closed. Do not leave any unused plates exposed to the environment.

Are SimPlates made of recyclable plastic?
The bottom portion of the plate is made of polystyrene #6 and is recyclable. The cover/lid is made of polyvinyl chloride and is not recyclable.

Tests & Accessories

Find the tests and accessories you need by selecting the link below.

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Resources & Product Information

Resources

IDEXX Water has reference materials and approval documents to support the many products in our water portfolio.

Product Information

SimPlate for HPC, unit-dose kit (25 tests)
Catalogue number: WHPC-25

SimPlate for HPC, multi-dose kit (100 tests, 10 media vessels and SimPlates)
Catalogue number: WHPC-100

SimPlate for HPC, media-only kit (40 tests)
Catalogue number: WHPC-40M

IDEXX Water Customer Support

UK Headquarters

IDEXX Technologies Ltd Units 1B & 1C, Newmarket Business Park, Studlands Park Avenue, Newmarket, Suffolk, CB87ER

Tel: 01638 676800

Wateruk@idexx.com

ISO Certifications

File Icon  ISO 9001:2008

File Icon  ISO 17025:2005

File Icon  ISO 14001:2004

1. Eaton AD, Clesceri LS, Greenberg AE, eds. Standard Methods for Examination of Water and Wastewater. 22nd ed. Washington, DC: American Public Health Association: 2012.

2. Data on file at IDEXX Laboratories, Inc.