
Alertys pregnancy tests
Establish a protocol for reproductive efficiency with superior accuracy.
- Reduce days open, increase production, and improve herd health with accurate, fast results.
- Our pregnancy tests are based on the detection of a panel of pregnancy-associated glycoproteins (PAGs), which are only produced in the presence of an embryo or fetus.
- Alertys milk- and blood-based tests offer flexible testing options with results as early as 28 days postbreeding.
Test with confidence

Alertys Milk Pregnancy Test
The Alertys Milk Pregnancy Test is an enzyme-linked immunosorbent assay (ELISA) for the detection of PAGs in milk samples.

Alertys Ruminant Pregnancy Test
The Alertys Ruminant Pregnancy Test is an enzyme-linked immunosorbent assay (ELISA) for the detection of PAGs in serum or plasma (EDTA).

Alertys Rapid Visual Pregnancy Test
The Alertys Rapid Visual Pregnancy Test is an enzyme-linked immunosorbent assay (ELISA) for the detection of PAGs in whole blood (EDTA), plasma (EDTA), or serum. This test can be performed without any lab equipment, in <30 minutes by series.
What test should I use?
Choose the Alertys test that's right for your herd
Dairy cows and heifers
Choose from the following tests based on your sample preference
Alertys Milk Pregnancy Test |
Alertys Ruminant Pregnancy Test |
Alertys Rapid Visual Pregnancy Test |
|
Sample type |
Milk |
Blood | Blood |
Testing location |
Laboratory | Laboratory |
On farm Through your veterinarian |
Part numbers |
99-41209 (5/strip) 99-41519 (30/strip) |
99-41169 (5/strip) | 99-41369 (2/strip) |
Beef cattle (and replacement dairy heifers)
Choose from the following tests based on your sample preference
Alertys Ruminant Pregnancy Test |
Alertys Rapid Visual Pregnancy Test |
|
Sample type |
Blood | Blood |
Testing location |
Laboratory |
On farm Through your veterinarian
|
Part numbers |
99-41169 (5/strip) | 99-41369 (2/strip) |
Sheep, goats, and water buffalo
Choose from the following tests based on your sample preference
Alertys Milk Pregnancy Test |
Alertys Ruminant Pregnancy Test |
Alertys Rapid Visual Pregnancy Test |
|
Sample type |
Milk |
Blood | Blood |
Testing location |
Laboratory | Laboratory |
On farm Through your veterinarian |
Part numbers |
99-41209 (5/strip) 99-41519 (30/strip) |
99-41169 (5/strip) | 99-41369 (2/strip) |
Bison
Choose from the following tests based on your sample preference
Alertys Ruminant Pregnancy Test |
|
Sample type |
Blood |
Testing location |
Laboratory |
Part numbers |
99-41169 (5/strip) |
When to test your herd
Initial pregnancy diagnosis
- 28 days postbreeding for dairy cows, heifers, beef cattle, and goats
- 29 days postbreeding for water buffalo
- 60 days postbreeding for sheep
Throughout gestation to confirm pregnancy status
- All
Pregnancy confirmation prior to dry period
- Dairy cows and heifers
Why use sample-based testing?
Use milk or blood samples to know if your cows or heifers are pregnant.
Alertys pregnancy tests use blood and milk samples to detect pregnancy-associated glycoproteins (PAGs), which are only produced in the presence of an embryo or fetus.
Complete list of species, sample types, and sensitivity for Alertys pregnancy tests
Assess overall herd health
An open pregnancy result can suggest the presence of disease.
Protect your herd from diseases shown to impact pregnancy rates, including Johne's disease, Neospora caninum, bovine vial diarrhea virus (BVDV), infectious bovine rhinotracheitis (IBR), and brucellosis.
Contact an IDEXX representative to learn more about Alertys pregnancy tests.
Frequently asked questions
- Label tube prior to sampling. Include date and individual cow ID. It is essential to ensure that the correct tube is used for the correct animal. Failure to do so may result in an incorrect status being assigned to the cow.
- Using the wet wipes or towel, remove dirt and detritus from the teats. Ensure udder is clean and dry prior to starting sample collection.
- Samples may be taken from individual quarters or as a composite sample of all four quarters.
- Discard a few streams of milk and remove the cap from the tube or vial. Angle tube at 45º to the teat end.
- Fill the tube or vial three-quarters full from one or multiple quarters and replace the cap.
- Place samples in a refrigerator prior to shipping to the laboratory.
- Samples should be well-packaged and kept cool during shipping (especially if preservative has not been used).
- Restrain the cow; clean the underside of the cow’s tail with a paper towel.
- Lift the cow’s tail straight up and measure up about 2 inches (7.5 cm) from the base of the tail, staying within the groove between the two bony ridges. This is the collection site.
- Insert the external needle into the underside of the tail, at the collection site, about 1/2 inch deep, keeping the needle perpendicular to the tail.
IMPORTANT: The external needle must be perpendicular to the tail. Do not angle the needle up, down, or to the side. - Push the tube in gently until the internal needle punctures the tube stopper. The vacuum will draw the blood into the tube. Note: If no blood appears, pull the external needle back slightly (without withdrawing it from the skin), and reinsert it in a different direction until you puncture the vein.
- When the tube is at least half full, withdraw the needle from the vein and remove the tube from the needle holder.
- Discard the needle and yellow/gray caps into a sharps container. The needle holder can be reused if undamaged.
- Make sure the tube label is clean and dry; label the tube with the sample number and the cow ID.
- For tests on milk: contact your regional milk lab or your DHIA
- For tests on blood: contact your vet or your regional lab
Please contact your regional lab or contact an IDEXX representative near you.
Most test kits require a sample tube for blood or milk collection.