24-hour detection of enterococci


  • Accurate results overnight so you can act quickly to protect the public and public resources.
  • Discover enterococci contamination in half the time of standard methods.
  • Eliminate tedious membrane filtration work.

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  • No media preparation.
  • No autoclaving.
  • No colony counting.
  • No glassware cleaning.
  • Less than 1 minute of hands-on time.
  • Results in 24 hours rather than 48 to 72 hours.
  • Sensitive to 1 enterococci per 100 mL.
  • Enumerates up to 2,419 enterococci per 100 mL without dilutions (with Quanti-Tray/2000).
  • Less subjective interpretation.
  • 50% fewer false positives and 95% fewer false negatives than the standard membrane filtration (MF) method.1
  • Up to 12-month shelf life minimises waste.
  • 24-hour test saves incubator space.


How the Enterolert Test works

The Enterolert Test uses a proprietary Defined Substrate Technology (DST) nutrient indicator to detect enterococci. This nutrient indicator fluoresces when metabolised by enterococci. DST improves accuracy and avoids the need for hazardous sodium azide suppressants used in traditional media.

How to use


How to use the Enterolert Test


Step 1

Add reagent to sample.

Step 2

Pour into Quanti-Tray (counts from 1–200) or Quanti-Tray/2000 (counts from 1–2,419).

Step 3

Seal using Quanti-Tray Sealer and incubate for 24 hours at 41°C ± 0.5°C.

Step 4: Quanti-Tray

For counts from 1–200: Count fluorescent wells and refer to most probable number (MPN) table.

Step 4: Quanti-Tray/2000

For counts from 1–2,419: Count fluorescent wells and refer to most probable number (MPN) table.

Frequently asked questions


The Enterolert test can be used as a method for enterococci detection in following types of water:

  • Ambient waters—includes fresh, marine, or estuarine surface water used for recreation, propagation of fish, shellfish, or wildlife; agriculture; industry; navigation; or as source water for drinking water facilities
  • Ground water
  • Wastewater

Some species of enterococci that Enterolert Test detects are: faecalis, faecium, avium, gallinarum, casseliflavis, and durans.

Enterolert Test detects enterococci at 1 organism/100 mL.

The shelf life of the Enterolert Test is up to 12 months from the date of manufacture.

The Enterolert reagent powder should be tan in colour; it should be dry and free-flowing. Any concerns about the colour or integrity of the powder should be directed to IDEXX Technical Services.

Yes, the Enterolert Test can be run with Quanti-Tray, Quanti-Tray/2000 or any multiple-tube format.

There is no comparator for the Enterolert Test. For comparison, use a negative control when interpreting results.

Check with your local authority for proper disposal of bacteriological biohazard materials at your facility.

Resources & Tools

Tests & Accessories

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Product Information

Enterolert Test (20-test pack)
Product Number: 98-21374-00
Catalogue Number: WENT020

Enterolert Test (200-test pack)
Product Number: 98-21375-00
Catalogue Number: WENT200

IDEXX-QC Enterococci Kit
Product Number: 98-29002-01
Catalogue Number: WQC-ENT

Snap pack and Quanti-Tray combination packs are also available.


IDEXX Water has reference materials and approval documents to support the many products in our water portfolio. Find the document(s) you need by selecting the link below.

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Water Customer Support

UK Headquarters
IDEXX Technologies Ltd Units 1B - 1D
Newmarket Business Park
Studlands Park Avenue
Newmarket, Suffolk, CB8 7ER

Tel: +44 (0) 1638 676800

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ISO Certifications

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1.  Budnick GE, Howard RT, Mayo DR. Evaluation of Enterolert for Enumeration of Enterococci in Recreational Water. Appl Environ Microbiol. 1996;62:3881–3884.